Information for Peptide: AAGDEFETR from B2CL2_HUMAN

1. Biological Peptide

1.1 Native Sequence

    R.AAGDEFETR.F

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
48	56	994.44	498.23	332.49	4.14

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
A	b		y
A	b		y
G	b		y
D	b		y
E	b		y
F	b		y
E	b		y
T	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

AAGDEF(c9n1)ETR (Modification: Stable Isotope on F6 (U-13C9, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1004.46	503.24	335.83	4.14

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
A	b		y
A	b		y
G	b		y
D	b		y
E	b		y
F	b		y
E	b		y
T	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for AAGDEFETR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y4	562.31	20.0
y5	691.35	21.0
y6	806.37	20.0
y7	863.4	19.0
y8	934.43	22.0

2.6 Calibration Curve of Standard

Description: A dilution series was done on the internal standard from 50 fmol to 0.1 fmol. Sensitivity was found to 0.1 fmol.

 

Experiment Description: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides