Information for Peptide: AAFFVLLPER from BOK_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.AAFFVLLPER.-

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
203	212	1161.65	581.84	388.23	6.05

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
A	b		y
A	b		y
F	b		y
F	b		y
V	b		y
L	b		y
L	b		y
P	b		y
E	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

GAFFVLLPER (Modification: A1 to G )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1147.64	574.83	383.55	6.0

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
A	b		y
F	b		y
F	b		y
V	b		y
L	b		y
L	b		y
P	b		y
E	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for AAFFVLLPER

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y7	873.52	19.0
y6	726.45	21.0
y5	627.38	20.0
y4	514.3	19.0
y3	401.21	17.0

2.6 Calibration Curve of Standard

Description: A Dilution series was done on the internal standard from 50 fmol to 10 fmol. The sensitivity was determined to be 10 fmol.

 

Experiment Description: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides